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For co-immunoprecipitation assays, human IR, IR Y972F and IR L973F cDNA were cloned into 3 Flag-CMV-14 mammalian expression vector (Sigma)

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Previously /the authors/ reported that isoflavone (genistein) activated bone sialoprotein (BSP) gene transcription is mediated through an inverted CCAAT box in the proximal BSP gene promoter